Your skin knows how to renew itself. Sometimes it just needs the right ingredients — delivered to exactly the right place. Topical skincare can only reach so far. The real work of collagen, elastin, and hydration happens deeper — in the dermis, where fibroblasts live.
The NCTF 135 HA Program delivers 60 bioactive ingredients precisely to that level, creating the conditions for your skin to rebuild from within. Not a filler program. Not a surface treatment. Something more fundamental. Here’s everything you need to know to decide if it’s right for you.
The NCTF 135 HA Program is a polyrevitalizing injectable solution containing 60 bioactive ingredients — including non-crosslinked hyaluronic acid, 24 amino acids, 12 vitamins, 6 minerals, 6 co-enzymes, 5 nucleosides, and glutathione — delivered through micro-injections directly into the dermis to stimulate fibroblast activity, boost collagen and elastin production, restore deep hydration, and improve overall skin quality. Developed by FILLMED Laboratories (France) with over 40 years of clinical use worldwide.
What makes the NCTF 135 HA Program fundamentally different from simple hyaluronic acid skin booster programs is the complexity and intentionality of its formulation. Each ingredient group plays a specific role in fibroblast activation and collagen production:
(non-crosslinked, 5 mg/mL) — Hydrates and plumps the dermis by retaining up to 1,000 times its weight in water. Unlike crosslinked HA filler programs, this form integrates into the skin's existing matrix without adding volume.
— The building blocks of proteins, essential for collagen and elastin synthesis. Four are particularly critical: Glycine, Proline, Hydroxyproline, and Lysine — the key amino acids that form the triple-helix structure of collagen molecules. Without an adequate supply at the dermal level, fibroblasts cannot produce collagen efficiently — regardless of how strongly they're stimulated.² ³
— Including Retinol (Vitamin A) for regulation of cellular activity and protection of collagen from MMP degradation; Ascorbic Acid (Vitamin C) for collagen fibre conversion; and Tocopherol (Vitamin E) for anti-inflammatory and photoprotective effects.
— Precursors for DNA and RNA synthesis, regulating the genes responsible for collagen and elastin production.
— Including NAD, NADP, and CoA — metabolic cofactors that drive the enzymatic reactions necessary for cell metabolism. Without them, fibroblasts lack the energy for protein synthesis.
— Magnesium, Sodium, Potassium, Calcium, Chloride, and Phosphate, supporting biochemical reactions including collagen production.
— One of the body's most powerful antioxidants. Neutralises free radicals and reduces oxidative stress — a major driver of premature ageing and fibroblast dysfunction.
Put simply: rather than stimulating the skin with energy (as lasers and RF devices do) or filling it with structural gel (as filler programs do), the NCTF 135 HA Program nourishes the skin at the cellular level — giving fibroblasts every ingredient they need to resume producing collagen, elastin, and hyaluronic acid naturally. Think of it as restocking a factory with raw materials, energy, and instructions.
The injectable skin booster market has grown significantly — with options ranging from simple hyaluronic acid hydrators to polynucleotide-based products. Here’s how the NCTF 135 HA Program is positioned differently.
Most skin booster programs contain 1–3 active ingredients — typically hyaluronic acid, sometimes with added vitamins or amino acids. The NCTF 135 HA Program contains 60 active ingredients across 7 functional categories, each designed to support a different aspect of fibroblast metabolism and skin renewal. This polycomponent approach is based on the scientific principle that ageing fibroblasts need multiple substrates simultaneously — not just one stimulus — to restore their biosynthetic activity.¹
A published review in Clinical, Cosmetic and Investigational Dermatology (Prikhnenko, 2015) concluded that the NCTF 135 HA Program’s complex formulation creates a favourable microenvironment for optimal fibroblast function — addressing energy supply (co-enzymes), raw materials (amino acids), genetic regulation (nucleosides), oxidative protection (glutathione/vitamins), and hydration (HA) simultaneously.¹
The NCTF 135 HA Program uses non-crosslinked hyaluronic acid, meaning it integrates into the skin’s existing moisture matrix rather than sitting as a discrete gel deposit. It doesn’t add volume, lift tissue, or change facial proportions. It improves the quality of the skin itself: hydration, density, firmness, radiance, texture, and fine lines.
This makes it ideal for patients who want their skin to look healthier and more luminous without any visible change in facial features — and for areas like the neck and décolletage where traditional filler programs are less appropriate.
The NCTF 135 HA Program has been used clinically since the 1980s, supported by multiple clinical trials (including the HEBE 2 and HEBE 3 studies), in vitro and ex vivo laboratory research, and a published 10-year comprehensive review covering cellular, histological, and clinical evidence.⁴ ⁵ ⁶
At BSL Clinic, we believe you deserve to know exactly what a treatment can — and cannot — do before you commit. Here’s what the research shows, presented without exaggeration.
In vitro studies demonstrate that the NCTF 135 HA Program produces statistically significant increases in fibroblast activity:
The fact that aged fibroblasts showed equal or greater response than normal fibroblasts matters: it confirms that ageing fibroblasts aren’t dead — they’re dormant, and they can be reactivated when given the right nutritional environment.¹
The HEBE 2 study (2019, randomised controlled, 10 centres, n=147) demonstrated that three sessions of the NCTF 135 HA Program significantly reduced superficial wrinkles on the face, neck, and décolletage versus anti-ageing cream alone — with statistically significant results at Day 75, maintained through Day 120.⁵
A separate 10-year review reported a 50% decrease in mean wrinkle score (P<0.0001), with significant improvements in skin tonicity, hydration, and radiance across five treatment sessions.⁴
Skin hydration increased significantly within 7 days of the final NCTF 135 HA Program injection in the HEBE 2 trial. Over 83% of patients reported enhanced skin hydration following treatment.⁵
The Grand-Vincent et al. clinical assessment (2017, n=40) measured outcomes after 5 sessions:
A 2024 prospective study (Nava-Castañeda et al., n=32) evaluated the NCTF 135 HA Program specifically for periocular skin quality. After three sessions, Face-Q satisfaction improved from 44.22 to 63.16 (P=0.0001), eye appearance from 41.22 to 57.00 (P=0.0001), and skin quality score from 11.52 to 5.31 (P=0.0001).⁷
A note from BSL Clinic: The NCTF 135 HA Program works progressively and cumulatively. The first session delivers an immediate glow and hydration boost — but the deeper, structural improvements in collagen density, firmness, and wrinkle reduction build over the full protocol of 3 sessions. This is a treatment that rewards patience and consistency.
The NCTF 135 HA Program is delivered through multiple micro-injections directly into the papillary dermis — the layer where fibroblasts reside. Using a fine needle (30G or 32G), your doctor places small deposits of solution approximately 1–1.5 cm apart, creating micro-papules that gradually diffuse into surrounding tissue.
Each injection point delivers approximately 0.05 mL of the solution, creating a localised zone of bioactive nutrients that fibroblasts can access directly. The non-crosslinked hyaluronic acid provides immediate hydration while the amino acids, vitamins, co-enzymes, nucleosides, and minerals create the metabolic environment for sustained collagen and elastin production.
The standard NCTF 135 HA Program consists of 3 sessions spaced 21 days apart — aligned with the skin’s natural cell renewal cycle. Each session reinforces and amplifies the previous one:
Maintenance treatments every 3–6 months sustain the effects long-term.
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The most popular treatment area. The NCTF 135 HA Program improves hydration, radiance, firmness, pore size, and fine lines across all facial zones — forehead, cheeks, perioral area, jawline, and chin. One vial is distributed evenly between both sides of the face per session.
The delicate skin around the eyes is among the first to show ageing and among the most difficult to treat safely with energy devices. The NCTF 135 HA Program has been specifically studied for periocular rejuvenation, showing statistically significant improvement in eye appearance satisfaction and skin quality.⁷ At BSL Clinic, we find that the eye area responds particularly well — delivering noticeable improvement in fine lines, dark circles, and crepey texture without the risks associated with ablative treatments in this sensitive zone.
These areas age visibly but are difficult to treat aggressively due to thinner skin and higher complication risk. The HEBE 2 study specifically included the neck and décolletage, confirming statistically significant wrinkle reduction in both areas.⁵
The dorsal hands lose volume and skin quality with age. The NCTF 135 HA Program improves skin hydration, thickness, and texture in the hands — complementing filler program–based volume restoration.
The NCTF 135 HA Program is also used for scalp mesotherapy to support hair follicle health and reduce hair thinning — though this is outside the scope of this skin-focused article.
The most robust clinical evidence for the NCTF 135 HA Program. Patients were assigned 3:1 to receive three NCTF 135 HA Program sessions (plus anti-ageing cream) or cream alone.
Results at Day 75 and Day 120:
Robin et al. compiled a decade of laboratory and clinical evidence:
Three sessions of the NCTF 135 HA Program produced significant improvement in periocular skin quality — Face-Q scores improving from 44 to 63 for facial appearance and 41 to 57 for eye appearance (both P=0.0001).⁷
Five sessions produced: 52% improvement in skin radiance (88% of participants), 33% wrinkle volume reduction (67%), 59% pore size reduction, and measurable increases in dermis density and thickness.⁶
Treatment outcomes from published peer-reviewed studies. Individual results may vary.
A note from BSL Clinic: The NCTF 135 HA Program is one of the most versatile treatments we offer — because it works with almost everything else. Whether you’re preparing for a laser resurfacing session, recovering from an energy-based treatment, or simply want your skin to look healthier and more radiant, this program can be tailored to fit your needs. A consultation will help us determine the best approach for your skin.
Minimal Downtime — Designed for Active Lifestyles
Mild redness, small papules, and slight swelling may appear. These are normal and typically resolve within a few hours to 24–48 hours.
Avoid touching the treated area, intense exercise, direct sun exposure, and makeup.
Most patients return to completely normal activities with no visible signs of treatment.
The NCTF 135 HA Program has been used clinically for over 40 years with a well-established safety record. The HEBE 2 study (n=147) reported no serious adverse events. Side effects are limited to the injection procedure itself — temporary redness, mild swelling, and small papules that resolve spontaneously.⁵
Allergic reactions are very rare but possible. Your doctor will review your medical history and any known sensitivities before treatment.
One of the NCTF 135 HA Program’s greatest strengths is its role as a facilitator of other aesthetic treatments. Published research supports its use in combination with both anti-wrinkle injections and calcium hydroxylapatite — with results that exceed what either treatment achieves alone.
Anti-wrinkle injections relax the muscles that create dynamic wrinkles — but they don’t improve the quality of the skin itself. The NCTF 135 HA Program addresses the other half of the equation: hydration, firmness, radiance, and structural integrity.
A published clinical study on forehead rejuvenation compared anti-wrinkle injections alone (one side) versus the NCTF 135 HA Program + anti-wrinkle injections (other side) in a split-face design. Results at Day 60:
The combination significantly improves skin quality and decreases wrinkles to a degree that is substantially superior to anti-wrinkle injections without the biorevitalization component.
At BSL Clinic, we commonly offer this combination — beginning with the NCTF 135 HA Program to optimize skin quality, followed by anti-wrinkle injections for dynamic wrinkle relaxation. This “inside-out” approach addresses both the skin and the muscle — for results that look natural and comprehensive.
This is one of the most innovative combination strategies in regenerative aesthetics — and it addresses a clinical observation that BSL Clinic finds particularly relevant: some patients respond less favourably to biostimulator treatments than others, and the reason may be a micronutrient deficit at the dermal level.
The concept of “skin priming” was introduced by Theodorakopoulou et al. (2023, Journal of Drugs in Dermatology), who hypothesised that delivering amino acids and co-enzymes via the NCTF 135 HA Program could create a nutritional reservoir that optimises the skin’s regenerative response to the CaHA Program. In other words: the CaHA Program tells fibroblasts to produce collagen, but the NCTF 135 HA Program gives them the raw materials and energy to actually do it.⁹
In a published case series, patients received a 1:1 mixture of CaHA (Radiesse Program) and NCTF 135 HA Program as a single treatment. Results after one session:
Mixing the NCTF 135 HA Program solution with CaHA at a 2:1 ratio increased pro-collagen Type I production by +49% compared to saline+CaHA (285.7 vs. 191.5 pg/mL) — confirming a synergistic effect at the molecular level.¹⁰
The CaHA Program works through mechanical stimulation — its microspheres activate fibroblasts via mechano-receptors. The NCTF 135 HA Program works through physiological stimulation — providing the 24 amino acids, 6 co-enzymes, and 12 vitamins that fibroblasts need for optimal protein synthesis. Combined, the mechanical signal and the nutritional support produce a “1+1=3” effect — greater collagen production than either treatment delivers alone.
Why BSL Clinic uses this approach: The skin priming concept is especially valuable for patients over 45, or those with chronologically mature skin where baseline fibroblast activity is significantly reduced. By priming the skin with the NCTF 135 HA Program — either mixed directly with the CaHA Program or delivered in a preceding session — we can enhance the collagen-building response and produce more consistent, visible results across a wider range of patients.
The NCTF 135 HA Program (New Cellular Treatment Factor) is a CE-certified polyrevitalizing injectable solution containing 60 bioactive ingredients — including non-crosslinked hyaluronic acid, 24 amino acids, 12 vitamins, 6 minerals, 6 co-enzymes, 5 nucleosides, and glutathione. It is injected into the dermis to stimulate fibroblast activity, boost collagen and elastin production, and restore deep skin hydration. Developed by FILLMED Laboratories (France) with over 40 years of clinical use.
Unlike filler programs, the NCTF 135 HA Program does not add volume or change facial proportions. Unlike simple skin booster programs that contain only HA, it delivers 60 active ingredients targeting every aspect of fibroblast metabolism — raw materials (amino acids), energy (co-enzymes), genetic regulation (nucleosides), antioxidant protection (glutathione/vitamins), and hydration (HA). It improves skin quality from within rather than adding external volume.
The standard protocol is 3 sessions spaced 21 days apart. Results are cumulative — each session builds on the previous one. Maintenance sessions every 3–6 months sustain improvement. For more mature skin, an initial intensive course of 4–6 sessions may be recommended.
Most patients notice an immediate glow and hydration boost after the first session. Progressive improvements in firmness, texture, radiance, and fine lines develop over the full 3-session protocol. Clinical studies show significant measurable improvement at Day 75 maintained through Day 120.⁵
The NCTF 135 HA Program is delivered through fine 30G or 32G needles. Most patients describe mild prickling — well-tolerated, especially with topical numbing cream. The procedure takes 15–45 minutes.
Minimal. Mild redness and small papules may be visible for a few hours to 24 hours. Most patients resume normal activities immediately.
Yes — and this is one of its greatest strengths. Published research supports combining the NCTF 135 HA Program with anti-wrinkle injections (significantly better wrinkle and skin quality outcomes) and with the CaHA Program (skin priming increases collagen production by 49%). It can also complement laser treatments, PRP, and other energy-based devices.
Face (full face including periorbital area), neck, décolletage, and hands. The HEBE 2 study validated efficacy on face, neck, and décolletage.⁵ A dedicated study confirmed periocular skin quality improvement.⁷
We use the NCTF 135 HA Program as part of a comprehensive skin strategy — not as an isolated treatment. Whether it’s priming your skin before a biostimulator, supporting recovery after laser resurfacing, or enhancing the results of anti-wrinkle injections, we integrate it into a personalized plan based on your specific skin needs.
Patients who are pregnant or breastfeeding, have active skin infections, known allergy to any component (particularly HA), or autoimmune skin conditions. A consultation determines suitability.
If you’ve read this far and want the short version — here it is.
The NCTF 135 HA Program has been studied for over 40 years, across lab settings, randomised controlled trials, and real patient assessments. The picture that emerges is consistent:
It activates dormant fibroblasts. Aged fibroblasts showed up to 148% more cell multiplication and 256% more collagen output in lab studies — not because they were failing, but because they were missing the right building blocks.¹
It reduces wrinkles — measurably. A 10-year clinical review found wrinkle scores dropped by 50% across five sessions. The landmark HEBE 2 trial confirmed significant improvement at Day 75, sustained through Day 120.⁴ ⁵
It works on the eye area. A dedicated periocular study showed Face-Q satisfaction scores jumped from 44 to 63 — a meaningful shift in how patients felt about how they looked.⁷
It makes other treatments work better. Combined with anti-wrinkle injections, wrinkle scores improved more than 4× versus anti-wrinkle injections alone. Combined with the CaHA Program, collagen production increased by 49% — confirmed in the lab.⁸ ⁹ ¹⁰
It works for most skin types, with minimal downtime. No serious adverse events in the HEBE 2 study (n=147). Most patients return to normal life the same day.⁵
This is not a treatment that works through one mechanism and hopes for the best. It works because it gives the skin everything it needs — simultaneously — to do what it was always designed to do.
The NCTF 135 HA Program isn’t about covering up what’s changed — it’s about giving your skin the biological tools it needs to function at its best again. Hydration, firmness, radiance, texture — these aren’t cosmetic additions. They’re signs of healthy, well-nourished skin that is actively producing the collagen, elastin, and hyaluronic acid it needs to look and feel vibrant.
If your skin has been telling you that something is missing — and topical products alone aren’t enough — the NCTF 135 HA Program may be the answer.
At BSL Clinic, the right treatment always starts with the right conversation.
Book a consultation. Let us assess your skin, explain what’s realistic, and design a plan that works for you.
Medically Reviewed by :
Wipanee Akarapusit, MD
Medical License No. 39678 — The Medical Council of Thailand
BSL Clinic, Bangkok
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30/8 Saladaeng Road, Silom, Bang Rak, Bangkok 10500, Thailand
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